Detection of Gaseous Ethylene Using Bodipy Ethylene Probes with 应用程序s in Plant Biology

Ethylene is a gaseous plant hormone that plays a large part in plant growth; by monitoring 和 detecting concentrations of gaseous ethylene, information on fruit ripening or flower growth can be provided 和 analyzed. This project proposes a gaseous ethylene detection system with applications in various fields, 比如植物生物学, 环境科学, 农业, 和园艺. Key objectives of the detector are accuracy, 稳定, speed of data retrieval, 易于操作, 和简单性. The project allows for an underst和ing of the most optimal detection set up that matches the objectives listed as closely as possible. The Michel Lab has developed several ruthenium based Bodipy Ethylene Probes (BEPs) based on olefin metathesis with varying kinetic 和 稳定 profiles, however these have almost exclusively been evaluated in solution to date. Initial evaluations will function similarly to the preliminary tests but will consist of evaluation of different substrates such as silica or variations of alumina, varying the amount of the probe used, adjusting spot sizes of substrate spotted on thin-layer chromatography (TLC) plates, 改变溶剂. 到目前为止, data has been collected on at least 8 variations of probes, 2溶剂, various probes amounts 和 concentrations, 和 different substrates ranging from TLC plates to printer paper. The probes tested so far include BEP-4, SIPr-BEP-4, Neo-BEP-4, Phenoxy-BEP-4, 2-Ad-BEP-4, Con-BEP-4, SIPr-Ox-BEP-4, 和Ox-NF5-BEP-5.